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Void volume is the amount of space in a very column that may be occupied by solvent. It's the space inside the column that is certainly outside of the column's inside packing substance. Void volume is measured on the chromatogram as the 1st ingredient peak detected, which is often the solvent that was present during the sample mixture; Preferably t
The separation basic principle in SEC is predicated over the completely, or partially penetrating on the higher molecular pounds substances from the sample into the porous stationary-section particles throughout their transport through column. The cell-period eluent is selected in this type of way that it fully stops interactions Along with the sta